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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochem Pharmacol. 2013 Sep 7;86(11):10.1016/j.bcp.2013.08.063. doi: 10.1016/j.bcp.2013.08.063

Fig. 5.

Fig. 5

Cell viability testing by MTT assay. PK15/hENT cells were seeded at 5 × 103 cells were seeded in 96-well plates, treated with 10 μM test compounds or control and incubated at 37 °C in phenol-free MEM for 24 hours. Subsequently, 10 μl of 5 mg/ml methylthiazolyldiphenyl-tetrazolium bromide (MTT) in PBS was added to each well, and then incubated for 4 hrs at 37°C. Following incubation, 100 μl of 0.04N HCl in isopropanol was added for 3 hours at room temperature to solubilize the formazan produced. The absorbance was quantified at 570 nm using a microplate reader (SpectraMax M2, Molecular Devices). Data are mean ± S.E.M for 4 replicates. * = (p<0.05). Compared to the cytotoxic agent camptothecin, compound 30 was significantly non-cytotoxic at p < 0.02.