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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Neurochem. 2013 Jun 25;126(4):10.1111/jnc.12298. doi: 10.1111/jnc.12298

Figure 1.

Figure 1

Choline increases ACh secretion by H82 SCLC cells. A. Choline significantly increased ACh secretion by H82 cells in a concentration-dependent manner. Addition of the cholinesterase inhibitor neostigmine (50 μM) resulted in increased levels of ACh in the medium. Secretion represent total amount of ACh secreted by 500,000 cells over 24 hours. B. There is a linear relationship between choline concentration and ACh secretion by H82 cells. Data are mean ± SE of 4 separate experiments. * p<0.05 versus no neostigmine; † p<0.05 versus 20 μM choline + neostigmine. C. Relative levels of ACh in cells versus medium. 500,000 H82 cells were cultured in standard medium (20 μM choline) in the presence of neostigmine for 24 hours and levels of ACh in media and cell pellet measured.