Skip to main content
. 2013 Dec 17;8(12):e82405. doi: 10.1371/journal.pone.0082405

Figure 4. Panels I, II and III represent immunostaining for PCNA (X200), cytokeratins AE1/AE3 (X200) and EGFr (X400) respectively of DU145 cells after fixation in 10% NBF fixation for 5 min, 684 hrs or 12 hrs in 10% NBF followed by transfer to 70% ethanol for 672 hrs.

Figure 4

The panels are 10% NBF fixation for 5 min (A), 10% NBF fixation for 5 min with AR (B), 10% NBF fixation for 684 hrs (C), 10% NBF fixation for 684 hrs with AR (D), 10% NBF fixation for 12 hrs plus 672 hrs in 70% ethanol (E) and 10% NBF fixation for 12 hrs plus 672 hrs in 70% ethanol with AR (F).