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. 2013 Dec 17;8(12):e84175. doi: 10.1371/journal.pone.0084175

Figure 1. Salinomycin induces autophagy in osteoblastoma cells.

Figure 1

Cultured U2OS osteoblastoma cells were treated with vehicle (0.1 % of DMSO), indicated concentration of salinomycin (Sali) or salinomycin (10 μM) plus 3-methyladenine (3-MA, 1 mM) for 24 hours, LC3B puncta fluorescence was detected by the confocal microscopy as described (A); the percentage of LC3B puncta positive cells was recorded (B), the expressions of LC3B, beclin-1, ATG-7, p62, β-actin and tubulin were detected by western blots (C). Cultured MG-63 cells were treated with vehicle or salinomycin (Sali, 1 and 10 μM) for 24 hours, LC3B puncta formation (D) and expressions of LC3B, beclin-1, ATG-7, p62, β-actin and tubulin were tested (E). Experiments in this figure were repeated three times. # p<0.05 vs. vehicle group. *p<0.05. Bar=15 μm (A).