Figure 1. γδ T cells modulate the production of Ang4.

(A) Expression levels of Paneth cell AMPs in the intestinal mucosa of naïve wild type (WT), TCRδ^-/- and TCRVγ1^‑/- mice as determined by RNA microarrays [82]. Levels are displayed as a ratio of the value obtained in TCRδ^-/- or TCRVγ1^-/- versus WT samples. (B) The level of Ang4 mRNA in the intestinal epithelium of TCRδ^-/- and WT mice was determined both prior to (NI) and 2h post-oral challenge and infection (I) with Salmonella Typhimurium by qPCR. Data (mean±SEM) are expressed relative to levels of β‑actin mRNA and were collated from 6 RNA samples in each group. (C) Ang4 protein levels in the intestine of naïve WT, TCRδ^‑/- and Vγ1^‑/- mice determined by immunoblotting. Membranes were stripped and re-probed with an anti-GAPDH antibody. The results shown are representative of those obtained using 4-6 mice of each strain. (D) Number of γδ⁺ and TCR-Vγ7⁺ iIELs in intestinal tissue sections by immunohistochemistry 6 weeks after transfusion of γδ iIEL-deficient mice with TCR-Vγ7⁺ or TCR-Vγ7^- iIELs. The data were collated (mean±SEM) by counting stained cells in at least 30 villi per section on a minimum of 5 sections per tissue from 4-6 mice. (E) Ang4 production is restored in iIEL-reconstituted TCRδ^‑/- mice to WT levels after transfusion of Vγ7⁺ (ATVγ7⁺) iIELs. Levels of Ang4, Ang1 and cryptidin 5 mRNA were determined by qPCR in samples of small intestine obtained from WT, TCRδ^-/- and TCRδ^-/- mice 6 weeks post‑reconstitution with Vγ7⁺ or Vγ7^- (ATVγ7^-) iIELs and 2h after oral challenge with Salmonella. Data (mean±STD) are expressed relatively to levels of β‑actin mRNA and were collated from RNA samples of 4-6 mice of each group.