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. 2013 Dec 17;8(12):e84075. doi: 10.1371/journal.pone.0084075

Figure 3. Bitter melon inhibited the recruitments of inflammatory macrophages into EAT and BAT in mice.

Figure 3

(A) Mac-2 immunostaining in EAT of mice; arrows indicate Mac-2+ macrophages; scale bars in (A), 100μm in length in low power views (×100) and 25μm in length in high power views (×400). (B) Quantification of Mac-2+ macrophage area in EAT, SAT and BAT of mice. (C) Real-time PCR quantitative mRNA expression of macrophage marker gene F4/80 in EAT, SAT and BAT of mice. Statistical difference between groups is shown using a nonparametric Mann-Whitney test. n=8 per group. *P<0.05, **P<0.01. All data in (B)-(C) are means ± SEM.