Table 2. Primers for quantitative real-time PCR.
| Gene | Sequence of forward primers(5' to 3',) | Sequence of reverse primers(5' to 3',) |
|---|---|---|
| F4/80 | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
| Itgax | CTGGATAGCCTTTCTTCTGCTG | GCACACTGTGTCCGAACTC |
| Inos | CCAAGCCCTCACCTACTTCC | CTCTGAGGGCTGACACAAGG |
| Chi3l3 | AGAAGGGAGTTTCAAACCTGGT | GTCTTGCTCATGTGTGTAAGTGA |
| Cd163 | CCTGGATCATCTGTGACAACA | TCCACACGTCCAGAACAGTC |
| mMcp-6 | GCCCAGCCAATCAGCG | CCAGGGCCACTTACTCTCAGA |
| Mcp-1 | CCCCAAGAAGGAATGGGTCC | GGTTGTGGAAAAGGTAGTGG |
| Il-6 | CCTTCCTACCCCAATTTCCAA | AGATGAATTGGATGGTCTTGGTC |
| Tnf-α | ACGGCATGGATCTCAAAGAC | AGATAGCAAATCGGCTGACG |
| Leptin | GAGACCCCTGTGTCGGTTC | CTGCGTGTGTGAAATGTCATTG |
| Ucp-1 | CACTCAGGATTGGCCTCTACG | GGGGTTTGATCCCATGCAGA |