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. 2013 Dec 17;8(12):e82871. doi: 10.1371/journal.pone.0082871

Figure 4. Expression of coilin decreases neurite outgrowth.

Figure 4

(A) PC12 cells were transfected with pEGFP or pCoilin-EGFP. After incubation in differentiation medium with nerve growth factor (NGF) for three days, neurite lengths were measured and their relative changes analyzed. (B) N-terminal EGFP-tagged wild-type and mutant coilin-constructs decreased the length of neurites significantly in the same experiment. For statistical evaluation, averaged relative neurite lengths of n>100 cells were compared, pooled from three individual experiments (means ± SEM; n.s.; A: **, p<0.01; Mann-Whitney test; B: n.s., non-significant; *, p<0.05; **, p<0.01; ***, p<0.001; Dunn’s test for comparison of multiple groups).