Figure 4. ERK2 dependent phosphorylation of PIMT increases PEPCK promoter activity.

293T were transfected with pGL3-PEPCK promoter and other constructs as mentioned in Figure 2 legend. Thirty hours post transfection, cells were lysed and luciferase activity was measured. The values were normalized with corresponding Renilla luciferase activity and expressed relative to PPARγ (unphosphorylated) (column 1) which was set to 1. Data are representative of 3 independent experiments. Statistical analysis was performed using one way ANOVA followed by Bonferroeni’s post hoc test. *p<0.001.