Figure 7. Overexpression of PIMT or Med1 increases hepatic PEPCK expression in mice.

Mice were injected via tail vein with Ad/LacZ or Ad/PIMT (A & B) or Ad/Med1 (C & D). After 5 days of injection mice were sacrificed and total RNA was isolated from liver. The expression of PEPCK was determined by northern blotting (A and C) or by qPCR (B & D). Blots were re-probed for GAPDH to ensure equal loading (A & C).