Figure 2. Effect of CLaudin-20 overexpression in human breast cancer cells. Confirmation of CLaudin-20 expression in MDA-MB-231(left) and MCF7 (right) cells (A). Increased invasive behavior of MDA-MB-231 (B) and MCF7 (C) cells overexpressing Claudin-20. Reduced adhesion to basement membrane of MDA-MB-231 (D) and MCF7 (E) cells overexpressing Claudin-20. Clausin-20 overexpression reduces transepithelial resistance (F) and paracellular (G) of MDA-MB-231 cells. Claudin-20 did not change attachment of MDA-MB-231 cells (H). MDA-MB-231 cells overexpressing Claudin-20 exhibited increased migratory phenotype (I). Imunofluorescent staining to show confirmation of Claudin-20 overexpression in MDA-MB-231 cells (J).