Figure 5. NDV-3 vaccination results in fewer phagocytes being recruited to the vagina in the later time points.

ICR female mice (n = 9 or 10 per group) were vaccinated SQ with NDV-3 (3 μg) or alum alone. Two weeks after the boost, mice were infected intravaginally with 1×10⁶ of C. albicans 529L. At the indicated time point, individually marked vagina was harvested, homogenized, and quantitatively cultured (A) and the MPO levels in the organ homogenates were measured by ELISA (B) Data are displayed as medians ± interquartile ranges. * P=0.03 vs. alum. Immunohistochemistry was performed on fixed sections of vagina using rat anti-mouse CEA antibody (neutrophils are stained in brown) (C) Magnification is 200x.