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(A) Overview of the homology model of HpPutP. The substrate binding site is enclosed by TMs 2, 3, 7, and 11, while the bound Na+ ion is coordinated by residues from TMs 2, 6, and 9. (B) The zoom-in view of the predicted Na+ and L-proline binding sites. The substrate and Na+ binding residues that have been mutated (see text) are shown in stick representation.
