Figure 2.

Muscimol produces opposing effects on behaviour. (a) Control and OA-exposed fish were placed in the shelter test arena and their location was recorded for 600 s. Two days later, muscimol (1 mg l⁻¹) was administered for 30 min prior to a second shelter test. The red lines illustrate the movement of one representative fish from each treatment over the trial. (b) There was no difference in time spent near the object for control (180 ± 80 s, n = 7) versus the OA-exposed fish (302 ± 82 s, n = 8; p > 0.05). Muscimol induced a significant difference in time spent near the object (control: 164 ± 98 s, n = 7; OA: 503 ± 60, n = 7; p < 0.05; Kruskal–Wallis test (p = 0.0382) with Dunn's Multiple Comparision post hoc test). Values are mean ± s.e.m. *p < 0.05.