Figure 1.

Dlx1/2 regulate the expression of Gucy1A3, Gucy1B3, and Nos1 (nNOS) in the developing basal ganglia. In situ RNA hybridization analysis of Gucy1A3 (A–D′, E–H′), Gucy1B3 (K–N′, O–R′), and Nos1 (S–V′) expression in the telencephalon of WT (left panels) and Dlx1/2^−/− (right panels) mice at E13.5 and E15.5. In each case, four planes of hemisections are shown (rostral to caudal). Gucy1A3 expression in the MGE is restricted to the dMGE. By E15.5, Gucy1A3 expression is detected in scattered cells in the cortical SVZ (higher magnification in I–J′ below); this expression is reduced in the Dlx1/2^−/− mutants (I′, J′, arrows). Dlx1/2^−/− mutants also have reduced Gucy1A3 expression in the SVZ of the LGE and MGE (E13.5 and E15.5); in addition, at E15.5, the mutants have ectopic Gucy1A3 expression in the caudoventral CGE/MGE (H′, E*, arrows), the region that has ectopic interneuron migration (Long et al., 2009b). Gucy1B3 and Nos1 expression is also greatly reduced in the basal ganglia anlage of the Dlx1/2^−/− mutants. CGE, Caudal ganglionic eminence; CX, cortex; GP, globus pallidus; Str, striatum. Scale bars: A–H′, K–V′, 500 μm; I–J′, 120 μm.