Table 1.
Proteins differentially expressed in CD133 low and CD133 high cells unambiguously identified after two-dimensional electrophoresis combined with mass spectrometry
| Gene symbol | Protein name | Swiss Prot. Ac. n° | Score | Coverage (%) | Peptides | MWt | Err ppm |
|---|---|---|---|---|---|---|---|
| TPM4 |
Tropomyosin alpha-4 chain (Tm4) |
P67936 |
651 |
33 |
10 |
29 |
6 |
| YWHAE |
14-3-3 protein epsilon (14-3-3 ϵ) |
P62258 |
116 |
22 |
2 |
29 |
7 |
| SFN |
14-3-3 protein sigma (14-3-3 σ) |
P31947 |
103 |
13 |
2 |
28 |
9 |
| YWHAB |
14-3-3 protein beta/alpha (14-3-3 β/α) |
P31946 |
433 |
35 |
9 |
28 |
8 |
| TCP1 |
T-complexprotein 1 subunit alpha (TCP-1-α) |
P17987 |
573 |
23 |
9 |
61 |
11 |
| SERPINB1 |
Leukocyteelastaseinhibitor (LEI) |
P30740 |
547 |
38 |
10 |
43 |
8 |
| SAHH |
Adenosylhomocysteinase (AdoHcyase) |
P23526 |
453 |
19 |
9 |
48 |
7 |
| EIF2S1 |
Eukaryotic translation initiation factor 2 subunit 1(eIF-2A) |
P05198 |
196 |
13 |
4 |
36 |
5 |
| PPP2CB |
Ser/threo-protein phosphatase 2A cat. sub. betaisof.(PP2A-β) |
P62714 |
125 |
22 |
2 |
36 |
4 |
| EIF3S2 |
Eukaryotic translation initiation factor 3 subunit 2 (eIF3β) |
Q13347 |
227 |
29 |
3 |
37 |
8 |
| DDAH1 |
NG-dimethylargininedimethylaminohydrolase 1 (DDAH-1) |
O94760 |
274 |
34 |
4 |
31.4 |
4 |
| PPA1 |
Inorganic pyrophosphatase (PPase) |
Q15181 |
281 |
30 |
6 |
33 |
8 |
| ANXA3 |
Annexin A3 |
P12429 |
549 |
34 |
10 |
36.5 |
4 |
| CAPZB |
F-actin capping protein subunit beta (CapZβ) |
P47756 |
351 |
30 |
6 |
31.6 |
7 |
| PSME3 | Proteasome activator complex subunit 3 (PA28γ) | P61289 | 122 | 11 | 3 | 30 | 3 |