Figure 3.

Expression of cone opsins and cGMP-gated cone channels is preserved in Gnb3^−/− cones. A, Immunostaining of WT and Gnb3^−/− retina for (from top) blue and green opsins, cone CNG channel subunits CNGA3 and CNGB3, and R9AP. For the R9AP staining, arrowheads point to cone outer segments. B, Representative immunoblots showing expression of cone opsins and CNGA3 (arrows) and β-actin for normalization in the WT and Gnb3^−/− retina. C, Bar chart showing relative staining intensities in the OS and total retina (Western blotting). Expression levels of these proteins are similar between the WT and null retina. The number of sets examined for the experiments have been indicated above the bars. Data are average ± SEM.