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. 2013 Dec 18;7:257. doi: 10.3389/fncel.2013.00257

FIGURE 2.

FIGURE 2

Denervation-induced homeostatic synaptic strengthening is not observed in granule cells of TNFα-deficient slice cultures at 3–4 dpl. (A) Mini-Ruby tracing of entorhino-hippocampal axons (red; ToPRO nuclear staining, blue) and electrical stimulations of the entorhinal cortex (EC) while recording evoked EPSCs from dentate granule cells revealed an intact and functional entorhino-hippocampal projection in slice cultures prepared from TNFα-deficient mice (TNFα-KO; three independent experiments each, up to 50 traces averaged per neuron). Evoked EPSCs (amplitude: 369 ± 102 pA) could be blocked by the AMPA-receptor antagonist CNQX (10 μM; amplitude: 9.6 ± 2.1 pA). Scale bar: 200 μm. (B) Patched granule cells were filled with biocytin and post hoc identified using Alexa568- or Alexa488-streptavidin. Scale bar: 50 μm. (C–E) Whole-cell patch-clamp recordings from granule cells of TNFα-deficient slice cultures revealed an increase in the mEPSC amplitudes at 1–2 dpl but not at 3–4 dpl (n = 12–16 neurons per group, from six to eight cultures each). Data represent mean ± SEM; ***p < 0.001; n.s., not significant.