Figure 11.

Calcium does not alter protein and lipid oxidative stress parameters. Using slot-blot assays, mitochondrial samples (200 ng/ml) were treated with increasing calcium concentrations and markers for protein oxidation (protein carbonyls), lipid peroxidation (4-HNE) and protein nitration (3-NT) and blot intensities assessed and expressed as percent increase compared to their levels in mitochondria without calcium. Protein carbonyls trended toward an increase at lower concentrations of calcium (100 and 200 nmol/mg mitochondrial protein) compared to higher concentrations (500 nmol/mg mitochondrial protein). However, these changes were not statistically significant compared to control (no calcium). No changes in the levels of 4-HNE or 3-nitrotyrosine (3-NT) were observed with any of the calcium concentrations used. Data represent group means ± s.e.m.; n = 3 per group; One-Way ANOVA.