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. 2013 Dec 18;5:10. doi: 10.3389/fnene.2013.00010

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Copyright © 2013 Pandya, Nukala and Sullivan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Calcium decreases mitochondrial respiratory control ratio (RCR). Increasing concentrations of calcium were used at the doses (0–1000 nmol/mg mitochondrial protein) as indicated and the RCR were calculated as State III (presence of ADP) over State IV (absence of ADP and presence of oligomycin). The mitochondrial RCR in the presence of calcium was significantly lowered with increasing concentrations of calcium when compared to the RCR of mitochondria with no calcium addition. Data represent group means ± s.e.m.; n = 3 per group. One-Way ANOVA followed by Fisher's post-hoc test; ^*p < 0.05 compared to 0 nmol/mg calcium.