Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 18;5:10. doi: 10.3389/fnene.2013.00010

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Pandya, Nukala and Sullivan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Calcium decreases mitochondrial bioenergetics in a time-dependent manner. Mitochondria (25 μg) were co-incubated with calcium after the addition of the complex I substrates (pyruvate and malate) for increasing amounts of time (1–6 min). An increase in the nmol of oxygen consumed upon addition of calcium was observed, while State III (ADP) driven respiration was inhibited with longer incubations with calcium (25 nmols/mg mitochondrial protein = 2.5 μM). When a higher concentration of calcium was added (100 nmols/mg mitochondrial protein = 10 μM), the inhibitory effect was more pronounced. However, as seen previously in Figure 5, calcium did not affect the complex II (Succinate) driven oxygen consumption. n = 2 per group.