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Expression of GAD1 in GAD1 knockdown cells. qRT-PCR shows that GAD1 mRNA expression in the shGAD1 cells (HSC2 and HSC3-derived transfectants; 2 clones each) are significantly lower than that in the mock cells (*P < 0.05, Mann–Whitney U test). Immunoblotting analysis shows that the GAD1 protein levels in shGAD1 cells (HSC2 and HSC3-derivrd transfectants; 2 clones each) have decreased markedly compared with that in mock cells.
