Skip to main content
. 2013 Dec 18;3:3545. doi: 10.1038/srep03545

Figure 2. Rho/Rock dependent actin modulation governs topography-induced changes in primary cilia structure.

Figure 2

(a) Alexa Phalloidin staining for f-actin (red) and nuclei stained with Dapi (blue) for MSCs cultured on flat and grooved surfaces with and without the Rho/ROCK inhibitor Y27632. Scale bar represents 10 μm. (b) Primary cilia length data for flat and grooved surfaces with and without Y27632 treatment. (+) represent statistical differences with respective flat group. (c) Primary cilia prevalence data for flat and grooved surfaces with and without Y27632 treatment. (d) representative ki67 staining showing one positive and one negative cell. Scale bar represents 10 μm. Prevalence of Ki67 positive staining and the influence of (e) surface topographies and (f) Rho/Rock inhibition by Y27632. (g) Primary cilia prevalence, with and without Rho/Rock inhibition with Y27632, on both topographies. Statistically differences denoted by + symbols are relative to respective flat topography group.