Fig.1.

Escape variant selections. Escape variant populations were selected from B6/K-0 cells or B6/K-0-derived subclones (arrows) by the SV40 T ag specific CTL clones H-1, Y-5, K-11 or Y-1. Two Y-1 selections and two K-11 selections were conducted (first, second). Numbers appearing in parentheses located on the arrows indicate, respectively, the total number of individual cultures used to begin each selection (multiple parallel cultures seeded with B6/K-0 cells, or cultures seeded with individual B6/K-0-derived subclone lines). The numbers or designations shown below a CTL clone represent designations given to the individual CTL resistant variant populations that emerged. Numbers set off by an angled line indicate a focus or island that was expanded from within a selection culture. The “p” designation for K-11 selected populations indicates that a secondary K-11 exposure (purge) was done for a portion of the resistant population following initial confluence of the primary selection culture. The identity of the B6/K-0 subclones used to seed selection cultures from which resistant populations emerged are indicated in parentheses beside the numeric designation for the corresponding resistant population.