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. Author manuscript; available in PMC: 2014 Dec 16.
Published in final edited form as: Chembiochem. 2013 Oct 24;14(18):10.1002/cbic.201300349. doi: 10.1002/cbic.201300349

Figure 4. Volatile sesquiterpene production by E. coli cultures expressing Stehi1|25180, Stehi1|64702 and Stehi1|73029.

Figure 4

The headspace of E. coli cultures expressing A) Stehi1|25180, B) Stehi1|64702 and C) Stehi1|73029 was sampled and analyzed for production of sesquiterpenes by GC/MS. The major product of cells expressing Stehi1|25180, Stehi1|64702 and Stehi1|73029 is Δ-6 protoilludene 7, a 1,11-cyclization of (2E,6E)-FPP. The minor peak observed for Stehi1|64702 and Stehi1|73029 was identified as β-elemene 8, which results from a 1,10-cyclization of (2E,6E)-FPP. Indole (labeled with an asterisk *), a breakdown product of tryptophan, is naturally produced by E. coli and serves as an internal standard.