Skip to main content
. 2013 Dec 18;4:394. doi: 10.3389/fmicb.2013.00394

Table 2.

Description and alpha-diversity estimation of the 16S rDNA pyrosequencing libraries of Sphagnum samplesa.

Libraryb Habitat Country Bog No. of raw seq. No. of filtered seq. No. of OTUs (97%) Chao1 Coverage (%) Shannon, H′
16S rDNA LIBRARIES SPECIFIC FOR Burkholderiac
AM1 S. magellanicum Austria Rotmoos 12,740 9,663 3.58 3.63 98.6 0.23
AM2 S. magellanicum Austria Wasenmoos 11,750 7,393 3.57 3.72 96.0 0.21
AM3 S. magellanicum Austria Pürgschachen Moor 13,271 9,612 4.45 4.53 98.2 0.24
AF1 S. fallax Austria Rotmoos 13,189 11,016 5.64 6.25 90.2 0.75
AF2 S. fallax Austria Wasenmoos 11,987 7,870 5.18 5.37 96.5 0.46
AF3 S. fallax Austria Pürgschachen Moor 13,843 7,595 4.25 4.27 99.5 0.38
RM1 S. magellanicum Russia Polesje 12,566 9,256 4.89 4.97 98.4 0.27
RM2 S. magellanicum Russia Polewoi mys 10,213 6,632 5.04 5.38 93.8 0.31
RM3 S. magellanicum Russia Oblojni moch 12,831 2,130 5.00 5.00 100.0 0.90
RF1 S. fallax Russia Polesje 13,051 1,788 3.00 3.00 100.0 0.79
RF2 S. fallax Russia Polewoi mys 10,279 7,637 4.40 4.64 94.9 0.26
RF3 S. fallax Russia Oblojni moch 13,304 7,325 5.22 6.44 81.1 0.33
16S rDNA LIBRARIES OF BACTERIA
RW Flark water Russia Oblojni moch 4,296 3,934 173.00 343.26 50.4 5.65
AFG S. fallax gametophyte Austria Pürgschachen Moor 5,399 2,869 252.00 591.94 42.6 6.47
AFS S. fallax sporophyte Austria Rotmoos 1,665 1,051 159.00 325.14 48.9 4.93
RFS S. fallax sporophyte Russia Polewoi mys 1,869 1,299 83.00 131.00 63.4 4.23
a

Richness estimates and diversity indices were calculated for the datasets normalized to the same number of sequences per library: 1,781 (Burkholderia), 1,051 (Bacteria).

b

Abbreviations specify the sampling sites and habitats: A, Austria; R, Russia; F, S. fallax; M, S. magellanicum; W, flark water; G, gametophyte; S, sporophyte. Arabic numerals specify different bogs in Austria and Russia.

c

16S rDNA pyrosequencing libraries specific for Burkholderia were obtained from gametophyte samples of Sphagnum mosses.

HHS Vulnerability Disclosure