Table 2.
Description and alpha-diversity estimation of the 16S rDNA pyrosequencing libraries of Sphagnum samplesa.
Libraryb | Habitat | Country | Bog | No. of raw seq. | No. of filtered seq. | No. of OTUs (97%) | Chao1 | Coverage (%) | Shannon, H′ |
---|---|---|---|---|---|---|---|---|---|
16S rDNA LIBRARIES SPECIFIC FOR Burkholderiac | |||||||||
AM1 | S. magellanicum | Austria | Rotmoos | 12,740 | 9,663 | 3.58 | 3.63 | 98.6 | 0.23 |
AM2 | S. magellanicum | Austria | Wasenmoos | 11,750 | 7,393 | 3.57 | 3.72 | 96.0 | 0.21 |
AM3 | S. magellanicum | Austria | Pürgschachen Moor | 13,271 | 9,612 | 4.45 | 4.53 | 98.2 | 0.24 |
AF1 | S. fallax | Austria | Rotmoos | 13,189 | 11,016 | 5.64 | 6.25 | 90.2 | 0.75 |
AF2 | S. fallax | Austria | Wasenmoos | 11,987 | 7,870 | 5.18 | 5.37 | 96.5 | 0.46 |
AF3 | S. fallax | Austria | Pürgschachen Moor | 13,843 | 7,595 | 4.25 | 4.27 | 99.5 | 0.38 |
RM1 | S. magellanicum | Russia | Polesje | 12,566 | 9,256 | 4.89 | 4.97 | 98.4 | 0.27 |
RM2 | S. magellanicum | Russia | Polewoi mys | 10,213 | 6,632 | 5.04 | 5.38 | 93.8 | 0.31 |
RM3 | S. magellanicum | Russia | Oblojni moch | 12,831 | 2,130 | 5.00 | 5.00 | 100.0 | 0.90 |
RF1 | S. fallax | Russia | Polesje | 13,051 | 1,788 | 3.00 | 3.00 | 100.0 | 0.79 |
RF2 | S. fallax | Russia | Polewoi mys | 10,279 | 7,637 | 4.40 | 4.64 | 94.9 | 0.26 |
RF3 | S. fallax | Russia | Oblojni moch | 13,304 | 7,325 | 5.22 | 6.44 | 81.1 | 0.33 |
16S rDNA LIBRARIES OF BACTERIA | |||||||||
RW | Flark water | Russia | Oblojni moch | 4,296 | 3,934 | 173.00 | 343.26 | 50.4 | 5.65 |
AFG | S. fallax gametophyte | Austria | Pürgschachen Moor | 5,399 | 2,869 | 252.00 | 591.94 | 42.6 | 6.47 |
AFS | S. fallax sporophyte | Austria | Rotmoos | 1,665 | 1,051 | 159.00 | 325.14 | 48.9 | 4.93 |
RFS | S. fallax sporophyte | Russia | Polewoi mys | 1,869 | 1,299 | 83.00 | 131.00 | 63.4 | 4.23 |
Richness estimates and diversity indices were calculated for the datasets normalized to the same number of sequences per library: 1,781 (Burkholderia), 1,051 (Bacteria).
Abbreviations specify the sampling sites and habitats: A, Austria; R, Russia; F, S. fallax; M, S. magellanicum; W, flark water; G, gametophyte; S, sporophyte. Arabic numerals specify different bogs in Austria and Russia.
16S rDNA pyrosequencing libraries specific for Burkholderia were obtained from gametophyte samples of Sphagnum mosses.