Fig. 4.

Effect of Gelidium amansii on the mRNA expression of NOX4. 3T3-L1 adipocyte were cultured during differentiation (for 8 days) treatments with NAC (10 mM), Gelidium amansii (1, 10, 20 and 40 μg/mL). Total cellular RNA was extracted on day 8 and performed RT-PCR. Gene expression of NOX4 was quantified and normalized with GAPDH. These data were measured as the standard deviation of three replicates. Means in the same column not sharing a common letter (a–e) are significantly different (p<0.05) based on Duncan’s multiple range test.