Fig. 6.

Effect of Gelidium amansii on the protein expression of GLUT4 and adiponectin. 3T3-L1 adipocyte were cultured during differentiation (for 8 days) treatments with NAC (10 mM), Gelidium amansii (1, 10, 20 μg/mL). Total cellular protein was extracted on day 8 and performed western blotting. Protein expression of GLUT4 and Adiponectin were quantified and normalized with GAPDH. Data are representative of three independent experiments, and GAPDH and target protein signals were from one representative blot. Individual antibodies were applied after stripping the membrane continuously. Less GAPDH amounts in control or NAC group were seems to be due to the technical error in loading. Means in the same column not sharing a common letter (a–c, a–b) are significantly different (p<0.05) based on Duncan’s multiple range test.