Fig. 1.

Schematic diagram of experimental protocol. Mice were sensitized on shaved flank skin with and without 50 μL of 10% phthalic anhydride (PA) on days 1∼3, 8∼10 and 15∼17. On day 29, 30, and 31, mice were challenged with or without 25 μL of 10% PA on the dorsum of both ears. Control group received only saline for 31 days. Experimental groups received the single dose of persimmon leaf extract (PLE) (250 mg/kg body weight) or EGCG (50 mg/kg body weight) one time per day for 31 days.