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Effect of BuOH extract of M. leucadendron L. on the LPS-induced phosphorylation and degradation of IκBα in RAW264.7 cells. Cells were pretreated with the indicated concentrations of BuOH extract of M. leucadendron L. for 1 hr before being incubated with LPS (10 μg/mL) for 24 hr. Cell lysates were then prepared and subjected to western blotting using an antibody specific for phospho-IκBα and IκBα. α-tubulin was used as an internal control.
