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. 2012 Mar;17(1):22–28. doi: 10.3746/pnf.2012.17.1.022

Fig. 3.

Fig. 3

Inhibition of NF-κB transcriptional activity by BuOH extract of M. leucadendron L. on LPS-stimulated RAW264.7 cells. The RAW264.7 cells transfected with an NF-κB reporter plasmid were treated with the indicated concentrations of BuOH extract of M. leucadendron L. and then stimulated with LPS (10 μg/mL). After 24 hr incubation, the activity of NF-κB was estimated by the luciferase assay. The relative NF-κB activity levels were expressed as the ratio of the activity of firefly luciferase over that of renilla luciferase. Data represent mean ±SD of three independent experiments performed in triplicate; *p<0.05 versus LPS alone.