Table 2.
Regression analysis of expression levels of specific proteins and age, HE staining intensity, protein nitrotyrosine levels, 4-HNE levels, heart weight, and TUNEL staining intensity of 6-, 26-, and 30-month female F344xBN hearts
| Age | Heart Weight | HE | Nitro-Tyrosine | 4-HNE | |
|---|---|---|---|---|---|
| Independent Variable | |||||
| Age | N.T. | 0.899*** (<0.001) | 0.965*** (<0.001) | 0.849*** (0.004) | 0.969*** (<0.001) |
| HE | 0.965*** (<0.001) | 0.802** (0.009) | N.T. | 0.767* (0.016) | 0.905*** (<0.001) |
| Heart Weight | 0.286 (0.455) | N.T. | 0.802** (0.009) | 0.911*** (<0.001) | 0.448 (0.226) |
| TUNEL | 0.690 (0.40) | 0.478 (0.193) | 0.722* (0.028) | 0.597 (0.090) | 0.718* (0.029) |
| OxyBlot | 0.672* (0.047) | 0.607 (0.083) | 0.688* (0.041) | 0.483 (0.188) | 0.682* (0.043) |
| Nitro-Tyrosine | 0.849** (0.004) | 0.911*** (<0.001) | 0.767* (0.016) | N.T. | 0.907*** (<0.001) |
| 4-HNE | 0.969*** (<0.001) | 0.924*** (<0.001) | 0.905*** (<0.001) | 0.907*** (<0.001) | N.T. |
| Apoptotic Signaling | |||||
| Bax | 0.263 (0.493) | 0.331 (0.385) | 0.111 (0.835) | 0.738 (0.094) | 0.478 (0.338) |
| Bcl-2 | 0.473 (0.199) | 0.356 (0.346) | 0.862* (0.027) | 0.394 (0.439) | 0.637 (0.174) |
| Casp-3 | 0.878** (0.007) | 0.928*** (<0.001) | 0.940** (0.005) | 0.887* (0.019) | 0.974*** (<0.001) |
| Casp-3 (19kDa) | 0.612 (0.08) | 0.664 (0.051) | 0.421 (0.406) | 0.863* (0.027) | 0.737 (0.094) |
| Casp-3 (17kDa) | 0.683* (0.043) | 0.740* (0.023) | 0.511 (0.301) | 0.894* (0.016) | 0.804 (0.054) |
| Akt | 0.409 (0.275) | 0.899*** (<0.001) | 0.236 (0.653) | 0.201 (0.703) | 0.052 (0.923) |
| p-Akt (Ser473) | 0.560 (0.117) | 0.336 (0.337) | 0.852* (0.031) | 0.372 (0.467) | 0.634 (0.176) |
*
The following symbols indicate (*) low correlation (p < 0.05), (**) moderate correlation (p < 0.01), (***) high correlation (p < 0.001), (†††) respectively. N.T. (not tested).