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. 2013 May;3(5):120137. doi: 10.1098/rsob.120137

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — (a,b) SSA3 and HSP26 transcripts detected in WT (BY4742pdr5Δ), rim15Δ, yak1Δ and rim15Δyak1Δ cells treated (a) with rapamycin or (b) with both rapamycin and MG132. (c,d) Normalized transcript levels of (c) SSA3 and (d) HSP26 to that of ACT1 and their level in WT cells at time 0 of treatment with rapamycin was set to an arbitrary value of 1. (e) Toxicity of GIS1 or MSN2 overexpression to cell growth on glucose (left) or ethanol/glycerol (right). GIS1 or MSN2 controlled by the tetO₇ promoter is switched on in the absence of doxycycline (Dox−) and off in the presence of the drug (+, 20 µg ml⁻¹).