Figure 7.

(a) The Rpn4 protein level in WT (pdr5Δ) cells treated with rapamycin (rap) and/or MG132. Endogenous RPN4 was tagged with myc in WT (pdr5Δ) cells. Mid-exponential cells were treated with rap (200 ng ml⁻¹) and/or MG132 (50 µM) for 0, 0.5 and 2 h. *Note that, in the original SDS-PAGE gels, the protein sample representing 2 h of treatment with both drugs was loaded into the well adjacent to the sample denoting 0 h of treatment with rapamycin. (b) PRE3 and RPT2 transcript levels in WT (pdr5Δ) and rpn4Δ deletion cells treated with rap and/or MG132 for 0, 0.5 and 2 h. (c) The doubling time of WT (pdr5Δ) and rpn4Δ deletion cells grown in SMM medium containing drug vehicle (V), rap (50 ng ml⁻¹), MG132 (12.5 µM) or both. (d) Rapamycin hypersensitivity of rpn4Δ deletion cells in YPD medium.