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Francisella tularensis SchuS4 O-antigen is expressed in E. coli DH5α cells. E. coli cells carrying the F. tularensis vector pGAB1 (containing O-antigen coding region) and empty pGEM-T Easy vector, respectively, stained with DAPI to visualize nucleic acid in blue (a,b) and probed with mAb FB11 and Alexa Fluor 488 conjugated secondary antibody (c,d). Images are shown at 100× magnification.
