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. 2013 May;3(5):130002. doi: 10.1098/rsob.130002

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — F. tularensis O-antigen is conjugated to ExoA by CjPglB in E. coli CLM24 cells. Two-colour immunoblots performed on His-tag purified ExoA using mouse mAb FB11 (green) and rabbit anti-6× His-tag antibody (red). Lane 1, E. coli CLM24 carrying pGAB2, pGVXN150, pGVXN115 (non-functional CjPglB control); lane 2, pGAB2, pGVXN150, pGVXN114 (functional CjPglB); lane 3, pGVXN150 only; panel 2b, close up view of His-tagged purified ExoA attached to various chain lengths of F. tularensis O-antigen. M = marker IRDye 680/800 protein marker. pGVXN150 contains ExoA, pGAB2 contains F. tularensis O-antigen, pGVXN114 and pGVXN115 carry a functional and non-functional CjPglB respectively.