Fig. 2. Comparison of Acyl-CoA Synthetases.
E. coli MHS04 (ΔfadR ΔfadD) harboring one of four acyl-CoA synthetase expression plasmids (medium copy, Ptrc) was fed dodecanoic acid. The rate of consumption was fastest for the strain expressing FadD. The control strain carried the empty pACYCtrc plasmid. The error bars represent standard deviations from biological triplicate shake flask cultures.
