Table 1.
Strains and plasmids used in this study
| Strain/Plasmid | Relevant Genotype/Property | Source or Reference |
|---|---|---|
| Strains | ||
| E. coli K-12 | F- λ- ilvG- rfb-50 rph-1 | ECGSC |
| MG1655 | ||
| E. coli DH10B | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG |
Invitrogen |
| E. coli DH5α | F- Φ80lacZΔM15 Δ(acZYA-argF) U169 recA1 endA1 hsdR17
(rk-, mk+) phoA supE44 λ- thi-1 gyrA96 relA1 |
Invitrogen |
| E. coli DY330 | F- λ rph-1 INV(rrnD, rrnE) ΔlacU169 gal490 pglΔ8 λcI857 Δ(cro-bioA) |
(Yu et al., 2000) |
|
Pseudomonas
putida KT2440 |
Source for PP_0763 | ATCC 47054™ |
| MHS01 | MG1655 ΔaraBAD ΔfadE Φ(PTrc-fadD) | This work |
| MHS02 | MG1655 ΔaraBAD ΔfadE::trcBTE | This work |
| MHS03 | MG1655 ΔaraBAD ΔfadE::trcBTE Φ(PTrc-fadD) | This work |
| MHS04 | MG1655 ΔaraBAD ΔfadR ΔfadD | This work |
| DE | MG1655 ΔaraBAD ΔfadE ΔfadD | This work |
| E | MG1655 ΔaraBAD ΔfadE | (Agnew et al., 2012) |
| RL08 | MG1655 ΔaraBAD ΔfadD | (Lennen et al., 2010) |
| TY19 | MG1655 ΔaraBAD ΔfadR ΔfadE::trcBTE | This work |
| TY27 | MG1655 ΔaraBAD ΔfadD ΔfadE::trcBTE | This work |
| TY30 | MG1655 ΔaraBAD ΔfadE::trcBTE ΔfadAB::trcBTE Φ(PTrc-fadD) | This work |
| TY31 | MG1655 ΔaraBAD ΔfadE::trcBTE ΔfadAB::trcBTE | This work |
| TY32 | MG1655 ΔaraBAD ΔfadR ΔfadE::trcBTE ΔfadAB::trcBTE | This work |
| TY33 | MG1655 ΔaraBAD ΔfadD ΔfadE::trcBTE ΔfadAB::trcBTE | This work |
| TY34 | MG1655 ΔaraBAD ΔfadE::trcBTE ΔfadAB::trcBTE ΔackApta::trcBTE Φ(PTrc-fadD) | This work |
| Plasmids | ||
| pBTRKtrc | Ptrc promoter, pBBR1 origin, KanR | This work |
| pUCtrc | Ptrc promoter, pUC origin, AmpR | This work |
| pACYCtrc | Ptrc promoter, pACYC origin, CmR | This work |
| pACYC-fadD | pACYCtrc carrying fadD under Ptrc control, CmR | This work |
| pACYC-PP0763 | pACYCtrc carrying PP_0763 (P. putida) under Ptrc control, | This work |
| CmR | ||
| pACYC-fadD6 | pACYCtrc carrying fadD6 (M tuberculosis) under Ptrc control, CmR | This work |
| pTrc99A | PTrc promoter, pBR322 origin, AmpR | (Amann et al., 1988) |
| ACR1 | pTrc99A carrying acr1 from Acinetobacter calCoAceticus under Ptrc control, AmpR | This work |
| FAR6 | pTrc99A carrying far6 from Arabidopsis thaliana under Ptrc
control, AmpR |
This work |
| ptrc99a-MAACR | pTrc99A carrying MAACR from Marinobacter aquaeolei under Ptrc control and fused to a maltose binding protein, AmpR |
This work |
| pBTRK-MAACR | pBTRKtrc containing MAACR | This work |
| pACYC-MAACR | pACYCtrc containing MAACR | This work |
| pUCtrc-MAACR | pUCtrc containing MAACR | This work |