Table 2.
Oligonucleotide primers used in this study
| Primer Name | Sequence (5’ to 3’) |
|---|---|
| 1. Forward rrnb | gaaaggttttgcaccattcgatggtgtCggtgcctaatgagtgagctaac |
| 2. Reverse before lacI | gaaaggttttgcaccattcgatggtgtCggtgcctaatgagtgagctaac |
| 3. Forward before lacI | atcgaatggtgcaaaacctttc |
| 4. Reverse from rrnb to get MCS, ptrc, and lacI |
gaaacgcaaaaaggccatcc |
| 5. Gibson MAACR fwd (MAACR gib F) |
acacaggaaacagaccatCACCAACAAGGACCATAGC |
| 6. Gibson MAACR rev (MAACR gib R) |
tcatccgccaaaacagcTTATCAGTGATGGTGATGATGG |
| 7. fadD fwd | gaaaagagctcggtaccAGGAGGTATAAGAAttgaagaaggtttggcttaacc |
| 8. fadD rev | gaaaagtcgactctagattaTCAGGCTTTATTGTCCACTTTGC |
| 9. BTEack-pta_int_F | atgttaatcataaatgtcggtgtcatcatgcgctacgctcGGCATGCGTTCCTAT TCCGAAGTTCC |
| 10. BTEack-pta_int_R | agcgcaaagctgcggatgatgacgagattactgctgctgtTACATCCGCCAAA ACAGCCAAG |
| 11. PP0763 fwd | GAGAAAgagctcggtaccAGGAGGTAAAATAATGTTGCAGAC ACGCATCATC |
| 12. PP0763 rev | GAAAAGcctgcaggtctagaTTAGTGATGGTGATGGTGATGCA ACGTGGAAAGGAACGC |
| 13. rev from start of MCS (ptrc gib R) |
atggtctgtttcctgtgtg |
| 14. fwd from end of MCS (ptrc gib F) |
gctgttttggcggatgag |
| 15. MAACR qPCR fwd | ctatgtctcctcgaaatc |
| 16. MAACR qPCR rev | gaatcgtagatcttggtg |
| 17. ompA qPCR fwd | tgttgagtacgcgatcactc |
| 18. ompA qPCR rev | gttgtccggacgagtgc |