Table 3.
The embryos quality and outcome of IVF-ET
| Characteristic | |
EFI |
r-AFS |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Total | ≤5 score | ≥6 score | z (χ 2 ) | P c | I-II stage | III-IV stage | z (χ2) | P d | |
| No. of cycles |
199 |
84 |
115 |
|
|
61 |
138 |
|
|
| No. of oocytes retrieveda |
8.0(4.0–12.0) |
6.0(3.0–10.8) |
9.0(5.0–14.0) |
−3.071 |
0.002 |
9.0(5.0–15.0) |
7.0(3.0–11.3) |
−2.454 |
0.014 |
| No. of fertilization |
5.0(3.0–9.0) |
4.0(2.0–8.0) |
6.0(4.0–10.0) |
−3.292 |
0.001 |
6.0(4.0–10.0) |
5.0(2.8–8.0) |
−2.493 |
0.013 |
| No. of fertilization 2PNa |
4.0(2.0-7.0) |
3.5(1.0-7.0) |
5.0(3.0–8.0) |
−2.735 |
0.006 |
5.0(3.0–9.0) |
4.0(2.0-7.0) |
−1.851 |
0.064 |
| Rate of 2PN fertilizationb |
1082/1834(59.0%) |
414/676(61.2) |
668/1158(57.7) |
2.232 |
0.135 |
379/671(56.5) |
703/1163(60.4) |
2.764 |
0.096 |
| Rate of polypronucleus zygote(%)b |
154/1834(8.4%) |
51/676(7.5) |
103/1158(8.9) |
1.012 |
0.314 |
70/671(10.4) |
84/1163(7.2) |
5.698 |
0.017 |
| No. of cleavage from 2PN |
4.0(2.0–7.0) |
3.0(1.0-6.0) |
5.0(3.0–7.0) |
−2.980 |
0.003 |
5.0(3.0–9.0) |
4.0(2.0–6.0) |
−2.082 |
0.037 |
| Cleavage rate from 2PN zygoteb |
1045/1236(84.5) |
391/465(84.1) |
654/771 (84.8) |
0.121 |
0.728 |
372/449(82.9) |
673/787(85.5) |
0.696 |
0.404 |
| Availability of embryos(%)b |
924/1306(70.8) |
347/496(70.0) |
577/810(71.2) |
0.242 |
0.623 |
310/475(65.3) |
614/831(73.9) |
10.862 |
0.001 |
| Top quality embryos(%)b |
139/1045(13.3) |
53/391(13.6) |
86/654(13.1) |
0.035 |
0.852 |
52/372(13.9) |
87/673(12.9) |
0.230 |
0.632 |
| No. Of embryos transferred |
2.0(2.0–2.0) |
2.0(1.0–2.75) |
2.0(2.0-2.0) |
−1.920 |
0.055 |
2.0(2.0–3.0) |
2.0(2.0–2.0) |
−2.349 |
0.019 |
| Implantation rate(%)b |
120/386(31.1) |
37/148(25.0) |
83/238(34.9) |
4.153 |
0.042 |
43/131(32.8) |
77/255(30.2) |
0.279 |
0.597 |
| Clinical pregnancy rate(%)b |
85/199(42.7) |
24/84(28.6) |
61/115(53.0) |
11.881 |
0.001 |
31/61(50.8) |
54/138(39.1) |
2.362 |
0.124 |
| Accumulation rate of pregnancyb | 107/199(53.8) | 33/84(39.2) | 74/115(64.3) | 12.266 | 0.000 | 37/61(60.7) | 70/138(50.7) | 1.678 | 0.195 |
Note: Rate of 2PN fertilisation = number of 2PN zygotes/number of oocytes retrieved; rate of polypronucleus zygotes = number of polypronucleus zygotes/number of oocytes retrieved; rate of cleavage from 2PN = number of cleavages from 2PN/number of zygotes; embryo availability rate = (number of frozen embryos + number of ET embryos)/number of cleavage-stage embryos; top-quality embryos = number of top-quality embryos/number of cleavage-stage embryos from 2PN; implantation rate = number of gestational sacs/number of ET embryos; clinical pregnancy rate = number of pregnancy cycles/number of embryo transfer cycles; accumulation rate of pregnancy = number of pregnancy cycles for fresh transferred embryos or freeze-thawed transferred embryos in a single cycle of ovulation stimulation/number of cycles for ovulation stimulation.
Definition of top-quality embryo: seven to eight blastomeres of equal size and less than 10% fragmentation can be seen on day 3 ( Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology, 2011) [19].
aThe non-normally distributed data are presented as median (25th-75th percentile). The non-parametric Mann–Whitney test was performed to calculate the Z-score.
bThe chi-squared test was performed to compare rates between groups.
cComparison between the EFI score ≤5 and ≥6 groups. (score ≤5 vs score ≥6).
dComparison between the r-AFS stage I-II and stage III-IV groups (stage I-II vs stage III-IV).