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. Author manuscript; available in PMC: 2014 Dec 17.
Published in final edited form as: Vaccine. 2013 Nov 6;32(1):10.1016/j.vaccine.2013.10.083. doi: 10.1016/j.vaccine.2013.10.083

Figure 3. Secreted levels of cytokines in the culture supernatants of spleen cells stimulated with rBmHATαc.

Figure 3

Cytokine levels (pg/ml) of (A) IL-4, (B) IL-10, (C) IFN-γ, (D) IL-1β, (E) IL-5 and (F) TNF-α were measured in the culture supernatants using an ELISA. Results show that significant level of IL-4, IL-10, IFN-γ, IL-1β and IL-5 were present in the culture supernatants of all vaccinated. Levels of TNF-α showed no significant differences between the groups. When AL007 or AL019 was used as the adjuvant the secreted levels of IL-4 was significantly higher compared to the other adjuvant groups. Data shown are OD normalized with the unstimulated controls. Each bar represents the mean ±SD levels of cytokines secreted by spleen cells. Significant levels of cytokines **(P<0.001) *(P<0.05) compared to control groups. Significant levels of cytokines +++ (P<0.05) compared to “no adjuvant” and also between the different adjuvant groups (One way ANOVA along with Tukey-Kramer post statistics test was used). Data is from one of two similar experiments showing comparable results.