Expression pattern of Delta and Notch homologs in embryos of the peripatopsid Euperipatoides rowelli. Light micrographs (A, B, D) and confocal laser micrograph (C). Anterior is left in B–D. Note the double-paired expression pattern of both genes along the ventral body surface, with a larger pair of posterior domains (arrows) and a smaller pair of anterior domains (arrowheads) in each body segment. (A) Overview of a late stage V embryo showing the expression pattern of Er-Delta. (B) Detail of the expression pattern of Er-Delta in the same embryo. (C) Expression of Er-Delta in a stage IV embryo, counterstained with the DNA marker Bisbenzimide. Note that the double-paired expression pattern of Er-Delta is already evident at this developmental stage. Also note that Er-Delta is expressed in a similar pattern in the jaw segment, whereas the fate of the ventral and preventral organs in this segment remains unclear. (D) Expression of Er-Notch in an early stage VI embryo. A weak expression is seen in a double-paired pattern, which is similar to the expression pattern of Er-Delta. Abbreviations: al, antennal lobe; an, presumptive antenna; ho, anlage of the hypocerebral organ; jw, presumptive jaw; lg, presumptive leg; sp, presumptive slime papilla. Scale bars: 400 μm (A), and 200 μm (B–D).