Table 1.

Rate constants of target proteins interacting with Trxh1.

Method	Rates	roGFP	MDH	PrxQ
				Rapid	Slow
SPRa	kon (M−1 s−1)	N.D.	247	7.97 × 104	765
	koff (s−1)	N.D.	3.01 × 10−4	0.62	5.62 × 10−4
Reduction reaction	Reduction rate (s−1)	N.D.	6.5 × 10−3b	0.67c

^aAssociation and dissociation rate constants were determined by using Trxh1_CS mutant.

^bReduction rate of MDH was determined from the reduction extent of MDH by wild type Trxh1 measured at the various reaction periods (for detail, see Materials and Methods).

^cReduction rate of PrxQ was determined from the amount of NADPH consumption in the presence of AtNTR, wild type Trxh1, PrxQ and hydrogen peroxide. The amounts of PrxQ was compensated to be a rate limiting step (for detail, see Materials and Methods).