Figure 2.

Expression of defense-related genes in leaves of lox3-4 mutant vs B73 NIL (WT) in response to C. graminicola infection. Approximately 3-week-old lox3-4 mutant and WT seedlings at 4 true leaf stage were infected with C. graminicola at 1 × 10⁶ spores.ml⁻¹.mlnicolaola with with at 4 tTrizol reagent and cDNA synthesized with Ambion 1st strand cDNA synthesis kit. PCR was conducted using standard procedures and the expression of GAPc was used as an internal control. HPL, hydroperoxide lyase; PAL, phenylalanine ammonia lyase; GST, glutathione S-transferase; MPI, maize proteinase inhibitor; PR1, pathogenesis related protein 1; GAPc, glyceraldehyde-3-phosphate dehydrogenase.