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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Leukemia. 2013 Mar 1;27(7):10.1038/leu.2013.66. doi: 10.1038/leu.2013.66

Figure 5.

Figure 5

Identification of a novel MHC class I-restricted NE peptide. (a) Epitope mapping for NE with the complete peptide library and minipools (A–P) by INFγ-ELISpot. (b) Peptide matrix for NE—each pool contains 8 15mer peptides. (c) Recognition of pools C and P, corresponding with recognition of peptide no. 59 in INFγ-ELISpot (mean ± s.d.). (d) Cytolytic activity of TAAmix CTLs against PHAB pulsed with NE no. 59 antigen or NE total pool (control: unpulsed PHAB, mean ± s.d.). (e) Blocking experiments with anti-HLA class I and II blocking antibodies using autologous peptide-pulsed PHAB as targets (mean ± s.d.). IFNγ-secretion was reduced to near background (unpulsed autologous PHAB) by preincubation with anti-HLA class I antibody, indicating MHC class I-restriction. (f) Intracellular cytokine staining of TAAmix CTLs stimulated with NE no. 59 showing predominant response in the CD8+ population.