Fig. 3.

Effects of oyster shell extract on NO levels in LPS-stimulated Raw 264.7 cells. Cells (2×10⁴ cells/well) in 96-well plates were first incubated with and without indicated concentrations of oyster shell extract for 2 h, and then incubated with LPS (1 μg/mL) for 20 h. Untreated is negative control without LPS treatment. Each value is expressed as mean±SD in triplicate experiments. Values with different alphabets are significantly different at p<0.05 as analyzed by Duncan’s multiple range test. OSE: Oyster shell extract.