Fig. 4.

Inhibitory effects of oyster shell extract on TNF-α, IL-1β and IL-6 production in LPS-stimulated Raw 264.7 cells. Equal amounts of cell lysates (30 μg) were subjected to electrophoresis and analysed for TNF-α, IL-1β and IL-6 production by Western blot. Raw 264.7 cells were preincubated with LPS (1 μg/mL) for 20 h, and then incubated without or with indicated concentrations of oyster shell extract for 20 h. Each value is expressed as mean±SD (n=3) and values with different alphabets are significantly different at p<0.05 as analyzed by Duncan’s multiple range test. OSE: Oyster shell extract.