Table 1.
Effects of oyster shell extract on antioxidant enzyme activities in LPS-stimulated Raw 264.7 cells
| Untreated | Oyster shell extract (μg/mL)+LPS (1 μg/mL) | |||||
|---|---|---|---|---|---|---|
|
| ||||||
| 0 | 10 | 25 | 50 | 100 | ||
| SOD (μM/mg protein/min) | 69.37±3.61a | 26.09±2.55e | 45.83±2.56d | 51.14±0.94c | 63.89±2.63b | 65.74±1.14ab |
| Catalase (μM/mg protein/min) | 1.66±0.04a | 1.24±0.01d | 1.56±0.01c | 1.59±0.01bc | 1.62±0.02ab | 1.63±0.03ab |
| GSH-px (unit/mg protein) | 4.26±0.01c | 2.71±0.04e | 3.41±0.04d | 4.25±0.03c | 4.57±0.02b | 5.21±0.03a |
Cells (2×104 cells/well) in 96-well plates were first incubated with or without indicated concentrations of oyster shell extract for 2 h and then incubated with LPS (1 μg/mL) for 20 h. Untreated is negative control without LPS treatment. Values with different alphabets are significantly different at p<0.05 as analyzed by Duncan’s multiple range test. SOD: Superoxide dismutase, GSH-px: Glutathione peroxidase.