Figure 3.

C5a induces production of IL-6 (A) and VEGF (B) from Müller cells. MIO-M1 cells were treated with 1 μM PGE₂ for 24 hours or left untreated. After washing, 0, 50, 100, and 200 ng/mL C5a were added, either in the absence or presence of 50 μM of C5aRA. Culture supernatants were collected 24 and 48 hours later, and levels of IL-6 and VEGF were measured by standard ELISA. Data are shown as mean ± SD, from representative results of four independent experiments. (*P < 0.05 compared with group without PGE₂ and C5a treatment.)