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. 2013 Dec 17;54(13):8191–8198. doi: 10.1167/iovs.13-12428

Figure 4.

Figure 4

Conditioned media from C5a-treated Müller cells promote human REC proliferation. Culture supernatants collected 48 hours after C5a stimulation from the above studies were added into hREC cultures (1:1 dilution), and REC proliferation was assessed 72 hours later using a CellTiter-Glo Luminescent Cell Viability Assay Kit (A). To verify the role of IL-6 and VEGF in the culture supernatant in promoting hREC proliferation, the experiments were repeated with IL-6 and VEGF neutralization mAbs (B). *P < 0.05.